Re:ancel® Cell Recovery Solution

Enzyme

  • Product Specification

    • Product Name

      Re:ancel® Cell Recovery Solution

    • Product Type

      Enzyme

  • Product Information

    • Product Detail

      Re:ancel® Cell Recovery Solution is a cellulase-based solution developed to degrade the nanocellulose network of Re:ancel® Bio, enabling simple recovery of cells, spheroids, and organoids from 3D culture while maintaining cell viability.

      Product Information

      • Product name: Re:ancel® Cell Recovery Solution
      • Product type: Cellulase-based solution
      • Formulation: 20 mg/mL cellulase mixture
      • pH: 6 (MES buffer)
      • Volume: 2.5 mL / vial
      • Storage: -20°C
      • Use: For research use only (RUO)

      Features

      • Target: Re:ancel® Bio 3D culture samples
      • Recoverable samples: Cells, spheroids, organoids
      • Treatment conditions: 37°C, overnight
      • Capacity: One vial digests approx. 8 mL of 1% Re:ancel® Bio
      • Cell viability: Maintained at approx. 90% or higher
      • Spheroid morphology: Maintained after recovery (HepG2)
      • Downstream applications: Protein extraction, RNA extraction, cell counting, imaging, plating
      • Recovery yield: Increased protein and RNA recovery compared with Re:ancel® Bio alone

      Workflow

      • Sample preparation → Media removal → Cell Recovery Solution treatment → Incubation at 37°C → Add PBS → Transfer to tube and centrifuge → Wash with PBS and centrifuge → Cell resuspension
    • Product Specification

      Cellulase

    • Appearance

      Transparent, Odorless